Figure 7.

HopF2 Arg-71 and Asp-175 Are Required for Virulence and ETI Functions.

(A) HopF2 Arg-71 and Asp-175 are required for its ability to induce nonhost HR in tobacco W38 plants. Tobacco leaves were infiltrated with the P. syringae pv tabaci strain 1152 containing the indicated constructs at 2 × 10⁸ colony-forming units/mL. Photographs were taken at 14 h after inoculation. The numbers on the side indicate areas showing HR/total injected areas. Infiltrated leaf areas are indicated by circles. The data shown are representative of two independent experiments.

(B) HopF2 Arg-71 and Asp-175 are required for virulence function in tomato plants. Tomato plants were infiltrated with the mutant strain lacking hopF2 (hopF2⁻), the hopF2⁻ strain complemented with the wild-type schF-hopF2 locus, or the hopF2⁻ strain complemented with the two mutant forms of schF-hopF2 at 5 × 10⁴ colony-forming units/mL. Leaf bacterial numbers were determined at the indicated time points (four replicates/data point). Error bars indicate sd. ** Significant difference compared with the hopF2⁻ strain carrying wild-type hopF2 at a P value <0.01 (Student's t test). The experiment was repeated three times with similar results.