Figure 1.

amsh3 Mutants Are Seedling Lethal and Accumulate Ubiquitin Conjugates.

(A) and (B) Phenotypes (A) and immunoblots (B) with an anti-ubiquitin P4D1 antibody from protein extracts of 7-d-old Arabidopsis seedlings overexpressing DEX-inducible AMSH3 (wild type [WT]) or AMSH3-AXA (AXA) grown in the absence (−) or presence (+) of 30 μM DEX. Seedlings with severe and weak phenotypes are shown for the DEX-treated AMSH3-AXA seedlings, which in strongly expressing transgenic lines represent up to 35 and 65% of the seedlings, respectively. CDC2 is a loading control. Bars = 1 mm.

(C) and (D) Phenotypes (C) and immunoblots (D) with an anti-ubiquitin P4D1 antibody from protein extracts of 7-d-old seedlings of amsh3-1, amsh3-2, and their trans-heterozygotes in comparison to wild-type seedlings of the same age. Bars = 1 mm.

[See online article for color version of this figure.]