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amsh3 Mutants Accumulate Autophagosomes.
(A) and (B) TEM image of amsh3 root epidermis cells after high-pressure freezing, freeze-substitution, and epon bedding. Arrowheads indicate autophagosomes. G, Golgi. Bars = 0.5 μm.
(C) Immunoblots of total protein extracts from wild-type and amsh3 seedlings using anti-ATG8e and anti-CDC2 (loading control) antibodies.
