Figure 3.

ONO Appears to Block the PIN Recycling Pathway.

(A) The effect of ONO on BFA compartment formation. PIN1- and PIN2-GFP signals (ProPIN2:PIN1/2-GFP) in root meristematic epidermal cells that were treated with BFA (50 μM) for 1 h ([a] and [d]) and washed out with half-strength MS solution ([b] and [e]) or with ONO (20 μM) ([c] and [f]) for 1 h or that were pretreated with ONO (20 μM) and BFA (50 μM) for 3 h ([g] and [i]) and washed out with half-strength MS for 1 h ([h] and [j]). Bars =10 μm.

(B) The effects of cycloheximide and auxin on PIN2-including ONO body formation. PIN2-GFP signals (ProPIN2:PIN2-GFP) in root meristematic epidermal cells that were pretreated with DMSO only (a), CHX (50 μM [d]), or auxin (NAA, 5 μM; [g]) for 30 min, pretreated as in (a), (d), and (g), respectively, and treated with ONO (50 μM [b]), CHX+ONO (50 μM for each [e]), or NAA+ONO (5 and 50 μM for each [h]) for 3 h, or pretreated as in (a) and (d), treated as in (b) and (e), and washed out with half-strength MS for 1 h ([c] and [f], respectively). Bars = 10 μm.

[See online article for color version of this figure.]