Figure 2.

Structure of the Orr^Ds Allele and the 5′ Regions Obtained by RACE Analysis in the Different Orr^Ds Genotypes and the Wild Type.

(A) The diagram displays the structure of the Orr^Ds allele. The ORR gene has two exons (black closed boxes) flanking one intron (black line between the exons). The position of composite Ds element (CDE) in the ORR 5′ end is marked. The CDE is composed of the CaMV 35S promoter flanked (downstream) by a complete Ds element and another element (possibly partial) upstream. ATG-I to ATG-IV are marked. At the bottom part, results of 5′ RACE analysis of the various Orr^Ds genotypes and the wild type (WT) are depicted. Long and short 5′ RACE products were obtained in the ex-35S-Orr genotype (ex-35S-Orr_L and ex-35S-Orr_S, respectively). The location of the corresponding Met along the amino acid (aa) sequence is indicated in parentheses next to each AUG codon. NRP, nested RACE primer. Black areas mark the putative protein formed in each transcript.

(B) The exact position of the CDE in the ORR gene sequence between ATG-I and ATG-II. The CDE and the three ATGs (I, II, and III) are marked by a bigger and different font.