Table 4.
The Most Altered Genes in the Heterozygous OrrDs Mutant Fruit
| Probe Set Name | OrrDs/OrrDs | OrrDsORRr | ex-35S-Orr | MapMan Bin | Annotation |
| Les.3652.1.S1_at | 5.8 | 6.0 | 0.1 | β-1,3 glucan hydrolases | β- 1,3-glucanase (SGN-U577505) |
| Les.5934.1.S1_at | 5.5 | 5.4 | −0.8 | Omega 6 desaturase | Omega 6 fatty acid desaturase (SGN-U574777) |
| Les.4829.1.S1_at | 3.4 | 5.1 | −1.4 | Ethylene synthesis degradation | E8 homolog (SGN-U579236) |
| LesAffx.56637.1.S1_at | 3.6 | 4.7 | −0.1 | Heat stress | DNAJ heat shock N-terminal domain-containing protein (SGN-U573193) |
| Les.4489.1.S1__at | 5.6 | 4.4 | 0.5 | Unknown | SGN-U578947, SGN-U590412, SGN-U579487 |
| Les.1175.2.S1_at | 5.5 | 4.2 | −0.1 | Signaling in sugar and nutrient physiology | PAR-1c (SGN-U569163) |
| Les.435.1.S1_at | 3.6 | 4.2 | -2.0 | Stress, biotic | Chitinase-endo (SGN-U566861) |
| LesAffx.70768.1.S1_at | 3.4 | 4.1 | −0.2 | AP2/EREBP, APETALA2/Ethylene-responsive family | DNA-binding protein (SGN-U573405) |
| Les.931.1.A1_at | −0.2 | 3.9 | 0.0 | WRKY transcription factor | WRKY (SGN-U589067, SGN-U573117) |
| LesAffx.10279.1.A1_at | 3.1 | 3.8 | 0.0 | Transport nucleotides | Purine permease (PUP1) (SGN-U568852) |
| LesAffx.18067.1.S1_at | −2.9 | −4.0 | −0.7 | Unknown | SGN-U566840 |
| LesAffx.30832.1.S1_at | −2.7 | −4.0 | −1.2 | Unknown | SGN-U576446 |
| Les.4530.1.S1_at | −3.4 | −4.0 | 0.0 | Cell wall modification | Xyloglucan endotransglucosylase-hydrolase (XTH9) (SGN-U577928, SGN-U581575) |
| Les.5038.1.S1_at | −3.5 | −4.2 | 0.2 | Unknown | SGN-U571966 |
| Les.4353.1.S1_at | −3.6 | −4.3 | 0.0 | Cell wall modification | Xyloglucan endotransglycosylase (XET1) (SGN-U579684, SGN-U579445) |
| LesAffx.11542.1.S1_at | −3.7 | −4.4 | −0.1 | Calcium signaling | Calcium-binding EF hand family protein (SGN-U566130) |
| Les.429.1.S1_at | −3.9 | −4.5 | 0.4 | Cell wall modification | Xyloglucan endo-1,4-β-d-glucanase (SGN-U581358) |
| Les.2038.1.A1_at | −4.8 | −4.5 | 0.2 | GDSL-motif lipase | Lipase/hydrolase, GDSL-motif (SGN-U568434) |
| LesAffx.63587.1.S1_at | −4.1 | −4.9 | −2.0 | AP2/EREBP, APETALA2/ethylene-responsive family | ERF (ethylene response factor) subfamily B-4 (SGN-U5864)39 |
| LesAffx.58308.1.S1_at | −4.5 | −5.8 | −1.0 | AP2/EREBP, APETALA2/ethylene-responsive family | CBF4-like, DREB subfamily A-1 of ERF/AP2 factors (SGN-U563215) |
The 10 most strongly up- and downregulated Affymetrix probes sets that could be assigned to an SGN unigene in the heterozygous OrrDs/ORR mutant compared to the wild type (breaker stage). The log2 fold changes of these probe sets in the homozygous OrrDs/OrrDs, the heterozygous OrrDs/ORR, and the ex-35S-Orr line in comparison to the wild type and their inferred MapMan classes and annotations are reproduced. Values in bold denote significant changes (P < 0.01 after false discovery rate control). The annotations were retrieved from SGN, where available manual annotation was reproduced; otherwise, the best BLAST hit against GenBank or Arabidopsis was imported from the SGN website.