Figure 8.

Expression of a Constitutively Active Allele of fuz7 Partially Bypasses the Need for Sho1 and Msb2.

(A) The indicated SG200 derivatives were inoculated into maize seedlings. Prior to infection, either arabinose (to induce fuz7DD expression) or glucose (to repress fuz7DD expression) was added to the inoculums to a final concentration of 1%. Twelve days after infection, symptoms were scored as described in the legend to Figure 2. Inoculated strains and glucose/arabinose supplements are listed below each column, numbers of infected plants (n) are given above each column, and the color and pattern code for disease rating is depicted on the right.

(B) Macroscopy and microscopy symptoms after infection with SG200Δsho1 Δmsb2 fuz7DD in the presence of glucose. A representative infected leaf is shown on the left 12 d after infection. Confocal microscopy performed as described in the legend to Figure 3A reveals poor colonization of plant tissue 6 d after infection (right panel).

(C) Macroscopy and microscopy symptoms after infection with SG200Δsho1 Δmsb2 fuz7DD in the presence of arabinose. A representative infected leaf 12 d after infection is shown on the left. Confocal microscopy performed as described in legend to Figure 3A reveals strongly enhanced colonization of plant tissue 6 d after infection (right panel).