Figure 5.

Regulation of MFT by GA Is Mediated by DELLA Proteins.

(A) Germination phenotype of the wild type and mft-2 treated with 10 μM ABA plus different concentrations of GA. Error bars denote sd.

(B) MFT expression determined by quantitative real-time PCR in wild-type and ga1-3 seeds mock treated or treated with 10 μM GA. All germinating seeds were collected 16 h after stratification. Error bars denote sd.

(C) MFT expression determined by quantitative real-time PCR in wild-type and various DELLA mutant seeds in ga1-3 background mock treated or treated with 10 μM GA. All germinating seeds were collected 16 h after stratification. penta indicates the ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 mutant. Inset shows the comparison of MFT expression between the wild type and penta mutants. Error bars denote sd.

(D) MFT expression determined by quantitative real-time PCR in ga1-3 rgl2-1 rga-t2 35S:RGL2-GR seeds. Seeds were treated with 30 μM DEX plus 30 μM cycloheximide (CYC) or MOCK (0.09% ethanol) plus cycloheximide under vacuum for 1 h. They were subsequently washed three times and collected 4 and 8 h after sowing on MS medium. Error bars denote sd.

(E) ChIP results showing the binding of RGL2-6HA to the MFT promoter. Primers used for the enrichment test are described in Figure 3D. Seeds were sown on MS medium and harvested 16 h after stratification for ChIP assays. A significant difference in comparison with the enrichment of a TUB2 fragment is indicated with an asterisk (P < 0.05, Student's t test). Error bars denote sd.

(F) Germination phenotype of ga1-3 and ga1-3 mft-2 treated with 1 μM GA. Error bars denote sd.

(G) Germination phenotype of ga1-3 rgl2-1 and ga1-3 rgl2-1 mft-2 in the absence of exogenous GA. Error bars denote sd.