Figure 1.

DNA and Protein Sequences and Expression of RAVL1.

(A) The B3-DNA binding domain spans from amino acids 86 to 195. The C-terminal region is rich in Pro and Ser residues. The two italicized 8-bp stretches indicated by arrows in the N- and the C-terminal regions represent duplicated sequences of Ds insertions of ravl1-1 and ravl1-2, respectively.

(B) qRT-PCR measured the relative amounts of RAVL1 mRNA. RAVL1 is expressed in all tissues examined. SA, shoot apices. Tissues were extracted from 1- to 2-week-old seedlings.

(C) Mature leaves of normal plants were separated into blades, collars, and sheaths. The relative amounts of RAVL1 (left graph) and BRI1 (right graph) mRNA were measured by qRT-PCR. 25S rRNA was used as control to normalize the expression data. Error bars are se of the means of three qPCR replicates.