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. 2010 Jun 25;22(6):1777–1791. doi: 10.1105/tpc.109.069575

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© 2010 American Society of Plant Biologists

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Figure 3. — Seedling Phenotypes of ravl1-1, bri1 d61-1, and RAVL1 Overexpressors and Correlated Transcript Levels of RAVL1, BRI1, D2, D11, and BRD1.

(A) Ten-day-old ravl1-1 and bri1 d61-1 seedlings are shown. WT, wild type.

(B) The expression levels of BRI1, D2, D11, and BRD1 were examined in ravl1-1 and bri1 d61-1 plants by qRT-PCR. 25S rRNA was used control to normalize the expression data. Error bars are se of the means of three qPCR replicates.

(C) Two-week-old seedling overexpressors of RAVL1 (OX) and the RAVL1-GFP fusion (OX-G) are shown. Based on phenotypic intensity, two independent lines (OX4 and OX5; OX-G2 and OX-G3) were chosen as representatives for the RAVL1 and RAVL1-GFP transformants, respectively.

(D) Expression levels of RAVL1 and BRI1 were determined in OX4, OX5, OX-G2, OX-G3, and wild-type seedlings by qRT-PCR. 25S rRNA was used control to normalize the expression data. Error bars are se of the means of three qPCR replicates.