Figure 1.
Moss Telomere Length Analysis.
(A) TRF analysis of telomeric DNA in P. patens isolates Gd (lane 2) and Vx-1 (lane 3). Molecular weight markers are shown on the left. Genomic DNA was digested with TruII and hybridized with a plant telomere-specific (TTTAGGG)4 probe. Arabidopsis DNA was used as a control (lane 1).
(B) Bal31 analysis of genomic DNA from P. patens isolate Gd. TruII digestion of genomic DNA was performed without prior Bal31 treatment (0 min) or after various incubation periods with Bal31 exonuclease. Asterisk indicates cross-hybridizing interstitial telomeric DNA band that is not sensitive to Bal31 digestion for up to 60 min.