Figure 5.

Increased G-Overhang Signals in Δpot1 Mutants.

(A) In-gel hybridization results using a G-strand specific probe (C₃TA₃)₄ to detect telomeric DNA under native (lanes 1 to 4) and denaturing (lanes 5 to 8) conditions in the wild type (wt) and Δpot1. T4 DNA Polymerase (a 3′→5′ exonuclease [Exo]) was added to reactions shown in lanes designated by (+).

(B) Quantification of the G-overhang signal in (A). Δpot1 signal intensity relative to the wild type is shown (error bars measured for n = 4, sd is ± 0.835).