Figure 6.

Accumulation of Chromosome Fusions in Δpot1.

(A) and (B) TF-PCR detects multiple instances of end-to-end chromosome fusions in Δpot1 moss but not in the wild type (wt). TF-PCR results with subtelomeric primers specific for chromosome arms A and B and DNA from three individual colonies of the wild type or Δpot1 (A). TF-PCR results with one subtelomeric primer specific for chromosome arm B (B).

(C) Increased incidence of chromosome fusions in Δpot1 cultures continuously regrown from a single protoplast. Top panel: Diagram of the experimental design. Bottom panel: TF-PCR results with DNA from wild-type (lane 1) and Δpot1 colonies (lanes 2-4). Δpot1 colonies were regenerated from a single cell through protoplasting once (lane 2), twice (lane 3), or three times (lane 4) (see asterisks in top panel). Arrow indicates a nonspecific PCR product detected in some wild-type samples. Molecular weight markers are shown on the left of each panel.