Table 1.
Strains and plasmids used in this study.
| Strain or Plasmid | Description | Source or Reference |
|---|---|---|
| E. coli strains | ||
| SM10 λPir | Conjugation competent strain | Miller and Mekalanos (1988) |
| SY327 λPir | Routine cloning | Miller and Mekalanos (1988) |
| DH5α | Routine cloning | Cambau et al. (1993) |
| BL21-DE | protein expression in E. coli | Studier and Moffatt (1986) |
| Yersinia pseudotuberculosis strains | ||
| IP2666 pYV (pIBI) | WT Yptb | Ivanov et al. (2005) |
| IP2666 pYV- | WT Yptb cured of the virulence plasmid | J.M Balada, et al. (2006) |
| ΔyscF | Deletion of yscF (codons 2–86) in IP2666 | Davis and Mecsas (2007) |
| ΔyopBD | Truncation of yopB and deletion of yopD in YPIII | L. Logston, unpublished |
| ΔlcrV | Deletion of lcrV (codons 19–326) in IP2666 | This study |
| ΔyopN | Deletion of yopN (codons 2–287) in IP2666 | Davis and Mecsas (2007) |
| Plasmids | ||
| pGEM-T easy | Cloning of PCR products; Apr | Promega |
| pDS132 | Suicide vector; Cmr | Philippe et al. (2004) |
| pTRC99A | IPTG-inducible expression vector; Apr | Amann et al. (1988) |
| pBAD33 | Arabinose-inducible expression vector; Cmr | Guzman, et al. (1995) |
| pGEX-5X-1 | GST fusion expression vector; Apr | Amersham |
| pTRC99-yscF | Expresses yscF from tac promoter | Davis and Mecsas (2007) |
| pTRC99-yscF L54V | Expresses yscF L54V mutant from tac promoter | This study |
| pBAD-yscF | Expresses yscF from araBAD promoter | This study |
| pBAD-yscF L54V | Expresses yscF L54V from araBAD promoter | This study |
| pGEX-yscF | GST-YscF fusion expression vector | Davis and Mecsas (2007) |
| pGEX-yscF L54V | GST-YscF L54V fusion expression vector | Davis and Mecsas (2007) |
| pFlag-YscG-YscE-His | Expresses YscG and YscE from tac promoter | Greg Plano, Unpublished |
| pTRC99-his yscN | Expresses His-tagged yscN from tac promoter | This study |
| pBAD33-his yscN | Expresses his yscN from araBAD promoter | This study |
| pGEX62-lcrV | GST-LcrV fusion expression vector | H. Dams-Kozlowska, unpublished |
| pGEX-yopN | GST-YopN fusion expression vector | This study |
| pTRC99-yopN | Expresses yopN from tac promoter | This study |
| pBAD-lcrV | Expresses lcrV from araBAD promoter | D. Harmon, unpublished |
| pDS132-yscF L54V | Suicide vector for introducing L54V mutation | This study |