Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 8;59(8):1937–1947. doi: 10.2337/db09-1064

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

PMC Copyright notice

FIG. 7. — Perk gene dosage modulates diabetic progression of the Akita mouse. A–E: Four Perk genotypes, including Perk^+/+ (“2”), Perk^+/− (“1”), βPerk;Perk^+/+ (“β2”), and βPerk;Perk^+/− (“β1”), were crossed to Ins2^+/Akita mice to assess glucose homeostasis and insulin in the resulting strains. The expression of Perk mRNA is elevated ∼80% above normal in βPerk;Perk^+/+ in β-cells. A: Progression toward overt diabetes in Ins2^+/Akita mutant is positively correlated with increasing Perk gene dosage. Blood glucose was measured at P23 and P28 in the same group of mice (β2: n = 11, β1: n = 11, 2: n = 45, 1: n = 42; *P < 0.05, **P < 0.005, ****P < 0.00005, *******P < 0.00000005). B: Random blood glucose levels measured in P17 and P23 Ins2^Akita/Akita mutant mice show that higher blood glucose levels are associated with an increase in Perk gene dosage (2: n = 6, 1: n = 10; *P < 0.05). C: Pancreatic insulin content decreases with increasing Perk gene dosage in the Ins2^+/Akita mice. The insulin content of Perk wild-type and heterozygous mice with the βPerk transgene (β1 and β2) were pooled (n = 12) and compared with mice without the βPerk transgene (1 and 2) (n = 12) and found to be significantly different (*P = 0.03). D: Pancreatic insulin content decreases with increasing Perk gene dosage in the Ins2^+/+ mice (β1 and β2, n = 10; 1 and 2, n = 8). The difference between mice with and without βPerk transgene is statistically significant (*P = 0.02). E: An increase in Perk gene dosage was found to lead to an ablation of in vivo GSIS in P28 Ins2^+/+ mice (β1 and β2: n = 12, 1 and 2: n = 11; **P < 0.005). P28 Ins2^+/Akita littermates demonstrated lower fasting serum insulin levels and impaired GSIS independent of Perk gene dosage (β1 and β2: n = 11, 1 and 2: n = 10).