Skip to main content
. 2010 Jun 3;59(8):1915–1925. doi: 10.2337/db09-0663

FIG. 4.

FIG. 4.

Genetic knockdown of Notch signaling components in human podocytes and HEK293 cells. A: Knockdown of Notch-1 in human podocytes was carried out using shRNA. Expression of the full-length Notch-1, NICD, and HIF-1α in response to different stimuli was determined by Western blot analysis in human podocytes. B: The relative expression of HES-1, VEGF, and nephrin mRNAs was assayed by RT-PCR. P < 0.05 for control shRNA in *normal and #HG medium. C: Knockdown of MAML-1 by shRNA in HEK293 cells. Protein expression of the MAML-1 and HIF-1α proteins in HEK293 cells in response to different treatments was examined by Western blot analysis. D: The relative mRNA expression of MAML-1, HES-1, VEGF, and nephrin was analyzed in the MAML-1 knockdown cells under normal or HG conditions. P < 0.05 for control shRNA in *normal and #HG medium.