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. 2010 May 18;59(8):1906–1914. doi: 10.2337/db09-1365

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© 2010 by the American Diabetes Association.

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FIG. 3. — Altered gluconeogenic and lipogenic gene expression in Ptpn2+/− mice. Eight to 10-week-old Ptpn2+/− and +/+ male mice were fed a high-fat diet for 15 weeks. Livers were harvested from (A and C) 4-h fasted mice, or (B and D) at the end of hyperinsulinaemic-euglycemic clamps and processed for quantitative (ΔΔCt) RT-PCR to measure the expression of (A and B) gluconeogenic genes Pck1 and G6pc, or (C and D) lipogenic genes Srebf1 and Fasn, with Gapdh used for normalization; similar results were attained when 18S was used for normalization. E: Ptpn2+/− and +/+ male mice were fed a high-fat diet for 15 weeks, livers extracted, fixed in formalin, paraffin embedded, and processed for histology (hematoxylin and eosin). F: Mice were fasted for 4 h, livers isolated, and triglyceride (TAG), diglyceride (DAG), and ceramide extracted and quantified. Results shown are means ± SE; *P < 0.05, **P < 0.01 by a two-tailed Student t test. (A high-quality digital representation of this figure is available in the online issue.)