Figure 3.

Comparison of MCIp microarray and MassARRAY EpiTYPER data. (a-c) Diagrams at the top show signal ratios of microarray probes for both independent experiments (donor A in blue, donor B in red) corresponding to their chromosomal localization. Typical DMRs are enriched in the hypomethylated fraction of one cell type and in the hypermethylated region of the other one, resulting in a mirror inverted image. Orange-colored zones indicate sequence regions validated via bisulfite conversion. Middle panels schematically present the chromosomal location of DMRs (orange boxes). Regions analyzed by MALDI TOF MS of bisulfite-converted DNA are indicated at the bottom. White circles represent detectable CpGs while grey circles (or grey boxes in the heat map below) show CpGs not measured by MS. Heat maps depict the methylation status of individual CpGs as shades of blue with each box representing a single CpG. Data of at least six independent donors were averaged.