Figure 5. The relationship between PKB inhibition and the mitoK_ATP channel.

The mitoK_ATP channel inhibitor 5-HD (10 μm) was used to determine if the mitoK_ATP channel is upstream or downstream of PKB. For this, PKB inhibitor XI was encapsulated during 5-HD pre-treatment prior to ischaemia/reoxygenation (I/R) of rat left ventricular myocardial slices and compared to PKB inhibitor XI alone, as shown in A. For comparison, ischaemic preconditioning (IP) was also encapsulated during 5-HD administration prior to I/R. In all cases tissue samples were analysed for CK release during the 120 min reoxygenation (B), and for necrosis (C) and apoptosis (D) at the end of 120 min reoxygenation. Data represent the mean ±s.e.m. from 6 independent experiments. *P < 0.05 vs. I/R alone group; †P < 0.05 vs. IP group.