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. 2010 Apr 19;588(Pt 12):2173–2191. doi: 10.1113/jphysiol.2010.190462

Figure 7. The effect of PKB and PI-3K inhibition on I/R-induced injury in non-diabetic and diabetic human myocardium.

Figure 7

Non-diabetic tissue (Aa) was pre-treated with PKB inhibitor XI (1 μm) or wortmannin (0.1 μm) prior to ischaemia/re-oxygenation (I/R) and compared to ischaemic preconditioning (IP). A similar protocol (Ab) was employed using diabetic human myocardium except with the inclusion of diazoxide (100 μm; mitoKATP channel opener) to induce pharmacological preconditioning. In all cases tissue samples were analysed for CK release during the 120 min reoxygenation (Ba and Bb), and for necrosis (Ca and Cb) and apoptosis (Da and Db) at the end of 120 min reoxygenation. Data represent the mean ±s.e.m. from 6 independent experiments. *P < 0.05 vs. I/R alone group.