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. 2010 Apr 19;588(Pt 12):2205–2218. doi: 10.1113/jphysiol.2010.187674

Figure 3. Expression of AQP2 variants in X. laevis oocytes.

Figure 3

A, functional evaluation of all AQP2 variants. Oocytes were injected with cRNA coding for either wild-type (WT, 1 ng) or mutated (V24A, R187C and K228E, 5 ng) AQP2 along with controls (Ctrl) and incubated for 72 h prior to testing for water permeability capacities (see Methods). Pf values are in 10−4 cm s−1 and represent 7–8 determinations per condition. Data are representative of 5–7 individual assays. The same oocytes were tested in Western blot using either total membranes (B, 1 oocyte per lane) or purified plasma membranes (C, 40 oocytes per lane) fractions. PDI detection was performed on the same material to confirm the quality of the purification procedure for plasma membrane in C. D, immunofluorescence labelling of all AQP2 in the same samples showing retention of R187C within intracellular stores as opposed to AQP2-wt, V24A and K228E.