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Oocytes injected with water (Ctrl), HA-AQP2-wt alone (WT, 1 ng) or with untagged mutants (+, 5 ng) were probed for overall AQP2 abundances in total membranes using α-AQP2 (A) or specifically for AQP2-wt in purified plasma membranes using α-HA (B).
