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. 2010 Apr 26;588(Pt 12):2255–2267. doi: 10.1113/jphysiol.2009.186643

Figure 8. Isolated mucin granules contain ATP and other nucleotides.

Figure 8

A, quantification of the amounts of ATP and total adenyl purine in each of the fractions collected in the second gradient was performed by etheno-derivatization and HPLC analysis. Data are expressed as the concentration of ATP (left axis) and total adenine-containing species (right axis); note that left and right axes represent different concentration ranges. B, quantification of the content of adenyl purine species (ATP, ADP, AMP and adenosine) in the total cell lysate and isolated mucin granule fraction (i.e. fraction 4) was performed by etheno-derivatization and HPLC analysis. Data are the average of four independent granule isolations and represent the percentage distribution of each species with respect to the total adenyl purine content in the fraction (mean ±s.e.m.). Note, the total adenyl purine mass in the mucin granule fraction represented <5% of the cell lysate content.