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. 2010 Apr 27;18(7):1346–1356. doi: 10.1038/mt.2010.61

Figure 3.

Figure 3

Copy number of integration and spectral karyotype of phiC31 integrase–modified cord-lining epithelial cells (CLECs). (a) Normal spectral karyotype in 296 of 300 metaphases of integrase-modified CLECs (upper panel). Rare chromosomal translocations (lower panel), t(7:13) (p21:q22) and t(1:19) (q25, q13.3), were each observed in 1 of 300 metaphases. A third translocation, t(1:18) (q25, q12), was observed in 2 of 300 metaphases. (b) Frequency distribution of copy number of integrated transgene in CLECs determined by fluorescence in situ hybridization of a fluorescein-labeled vector-specific probe. Representative image of integrated transgene (green signals) in DAPI-stained interphase nuclei of CLECs. Original magnification ×1,000. Bar = 5 µm.