Figure 2.

Time course of IL-10 ELISpot responses to canine FIX (cFIX) in dogs receiving AAV-2-cFIX vectors by ATVRX. Secretion of IL-10 from PBMCs after stimulation with peptides spanning the cFIX sequence organized in 19 pools. Samples were collected and analyzed at baseline, during IS and after IS discontinuation. Responses were considered positive when the number of spot-forming units (SFU) per million PBMCs were >50 and at least threefold higher than the media control (Med). All test conditions were assayed in triplicate. (a) Group A (1 × 10¹² vg/kg with IS); (b) Group B (3 × 10¹² vg/kg with IS); (c) Group C (8.5 × 10¹² vg/kg with IS); (d) Group D (3 × 10¹² vg/kg without IS). Baseline sample for J62 was not available; a sample obtained 1 week after vector delivery was analyzed instead. ATVRX, afferent transvenular retrograde extravasation; IL, interleukin; IS, immunosuppression; PBMC, peripheral blood mononuclear cell.