Figure 2.

Confocal immunofluorescence analysis of porcine coronary arteries. Cross sections of left anterior descending (LAD), left circumflex (LCx), and right coronary arteries (RCA) obtained from sham-operated or MR animals injected either with AAV.SERCA2a or with saline were analyzed by confocal immunofluorescence (red) with specific antibody; anti-SERCA2a and anti-eNOS. The media was identified by elastin autofluorescence (green). At least three animals were analyzed for each group. Bar = 50 µm. AAV, adeno-associated virus; EC, endothelial cells; eNOS, endothelial isoform of nitric oxide synthase; IEL, internal elastic lamina; M, media; MR, mitral regurgitation; SERCA2a, sarco/endoplasmic reticulum Ca²⁺-ATPase 2a.