Figure 7.

Effect of SERCA2a overexpression on histamine-induced calcium transient. Calcium responses with increasing histamine doses (1–10 µmol/l) in (a) control are presented as a mean of several cell (black trace) or as a single representative cell (hatched trace), and (b, c) Ad-S2a or Ad-βgal-infected HCAEC stimulated with histamine (1–10 µmol/l) in presence of extracellular calcium (100 µmol/l EGTA plus 300 µmol/l CaCl₂) are presented as a mean of several cell or as a single representative cell. Ad, adenovirus; βgal, β-galactosidase; EGTA, ethylene glycol tetraacetic acid; SERCA2a, sarco/endoplasmic reticulum Ca²⁺-ATPase 2a; HCAEC, human coronary artery endothelial cell.