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GNeo-QD525 uptake depends on heparan sulfate. (a) Wild-type Chinese hamster ovary cells, (b) mutant pgsA-745 deficient in heparan sulfate and chondroitin sulfate, and (c) mutant pgsD-677 deficient in heparan sulfate were incubated with 5 nmol/l GNeo-QD525 (black), QD525 (dark gray), or untreated (none, light gray area) for 2 hours under normal growth conditions. The cells were rinsed twice and lifted with trypsin/EDTA before being analyzed by flow cytometry.
