FIGURE 5.
Kinetic effects of knockdown of cPAcP on tyrosine phosphorylation of ErbB-2. LNCaP C-33 cells were transiently transfected with siPAcP-126 plasmid or vector alone and harvested at 30, 36, and 48 h after transfection. An equal amount of cellular lysates was separated by SDS-PAGE for Western blot analyses. cPAcP, the specific tyrosine phosphorylation (p) level of ErbB-2, and phosphorylation of p52Shc were detected by the corresponding Ab. The protein levels of ErbB-2, Shc, and α-tubulin were examined after the membranes were stripped. Similar results were obtained from three sets of independent experiments.