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Aminoacyl transfer of serine is fast relative to adenylate turnover. The [14C]seryl-adenylate-H73A ThrRS (8–10 μm) was prepared as described under “Experimental Procedures” and then rapidly mixed with tRNA (4–6 μm) under rapid quench conditions over the interval of 10 ms–1 s. Then the resulting curve was fit to a single exponential equation to derive the rate of aminoacyl transfer (ktrans). The error bars at each time point represent the mean ± S.E. from three independent experiments.
