FIGURE 2.

RNG140, as well as RNG105, binds directly to RNA and inhibits translation in vitro. A, recombinant GST, GST-RNG105, GST-RNG140, and GST-RNG140 deletion mutants were incubated with RNA isolated from rat brains. RNA bound to the recombinant proteins was extracted and quantified. FL, full length; ΔN, deletion of N terminus (from N-terminal end to NLS); ΔC, deletion of C terminus (from RGG box to C-terminal end); ΔNC, deletion of both N and C termini. n = 3, *, p < 0.05, Student's t test. Error bars are S.E. B, luciferase mRNA was translated in rabbit reticulocyte lysates in the presence of 0.03 or 0.3 μm recombinant GST, GST-RNG105, GST-RNG140, and GST-RNG140 deletion mutants. Translated luciferase protein was detected by SDS-PAGE. C, intensities of the bands in B were quantified, and translational repression activity of each recombinant protein (0.3 μm) was calculated. n = 3, *, p < 0.05, Student's t test. Error bars are S.E.