FIGURE 3.

The RNG140-induced granules are distinct RNA granules from RNG105-induced ones. A, A6 cells expressing RNG105-GFP or RNG140-GFP were stained by in situ hybridization with poly(dT) for mRNA detection or immunostained with anti-ribosomal protein S6 (RPS6), anti-G3BP, anti-RNG105, anti-FMRP, anti-DCP2, and anti-XRN1 antibodies. Scale bar, 10 μm. B, quantification of co-localization of RNG105-GFP or RNG140-GFP granules with the stains in A. Shown are percentages of RNG105-GFP or RNG140-GFP granule areas that overlap with the stains. n = 10. Error bars are S.E.