FIGURE 5.

RNG140 is highly expressed in the adult brain. A, immunoblotting of mouse brain (cerebrum) extracts with an antibody generated against RNG140. B, immunoblotting of mouse tissue extracts with anti-RNG105 and anti-RNG140 antibodies. Both RNG105 and RNG140 are most highly expressed in the brain (cerebrum and cerebellum). In cerebrum, the anti-RNG140 antibody detected two upper bands and a lower band in addition to the RNG140 band after long exposure to detection reagents, although the bands were much fainter with shorter exposure (cf. A). These bands may be RNG140 with some modifications or nonspecific cross-reactants highly expressed in the brain except the possibility that the bottom band is a splice form of RNG140 encoding a predicted 812-amino acid protein (protein ID ENSMUSP00000072165 on the Ensembl Web site). The lower band detected by RNG105 antibody in brain extracts is an RNG105 degradation product, and the band in skeletal muscle may be a nonspecific cross-reactant specifically expressed in skeletal muscle. C, extracts from adult cerebrum and cerebellum and from brain of embryonic day 17.5 embryo were immunoblotted with anti-RNG105 and anti-RNG140 antibodies. 30 μg proteins are loaded on each lane.