FIGURE 6.

The RNG140-localizing RNA granules in neuronal dendrites are distinct from the RNG105-localizing RNA granules. A–F, rat hippocampal slices were immunostained with anti-RNG140 antibody and co-stained with anti-RNG105 (A), anti-RPS6 (B), anti-FMRP (C), anti-staufen (D), anti-G3BP (E), or anti-PSD-95 (F) antibody. The lower panels in A and B show higher magnification of the boxed areas. G, rat hippocampal slices were immunostained with anti-RNG140 antibody and co-stained by in situ hybridization with poly(dT) for mRNA detection. The lower panels show magnified images of the boxed areas. Arrowheads denote co-localization. Scale bars, 10 μm. H, quantification of co-localization of RNG140 granules with the antibody or poly(dT) staining in A–G. Shown are percentages of RNG140 granules that overlap with the indicated antibody or poly(dT) staining. n = 5, **, p < 0.01, significantly different from the left five bars in Tukey-Kramer test. Error bars are S.E.