FIGURE 7.

Knockdown of RNG105 and RNG140 reduces dendrite length in cultured neurons. A, primary cultured neurons from mouse cerebral cortex were co-transfected with a GFP reporter and siRNA for control, RNG105, or RNG140. GFP images of transfected neurons are shown. Scale bar, 10 μm. B, quantification of dendrites in A. Shown are the number of primary, secondary, and tertiary dendrites and the total dendrite length per cell. Cultured neurons were also co-transfected with RNG105 siRNA or RNG140 siRNA with RNG105 or RNG140 for rescue experiments. n = 9, *, p < 0.05, **, p < 0.01, ***, p < 0.001, Student's t test. Error bars are S.E.