FIGURE 4.

Recombinant goldfish IFNγrel induces higher monocyte phagocytic responses compared with rgIFNγ. Goldfish monocyte cultures were treated with medium, rgIFNγ (100 ng/ml), or rgIFNγrel (1, 100 ng/ml) and phagocytosis assessed by FACS. A, representative phagocytosis histogram plots of cells from an individual fish treated with medium, rgIFNγ, or rgIFNγrel. B, mean + S.E. phagocytic response of monocytes obtained from cultures established from individual fish (n = 5) that have ingested 3 or more beads following treatment with medium, rgIFNγ (100 ng/ml), rgIFNγrel (1, 100 ng/ml), a combination of rgIFNγ (100 ng/ml) and rgIFNγrel (100 ng/ml), or rgIFNγrel (100 ng/ml) in conjuction with 5 μg/ml of α-rgIFNγrel IgG. Statistical analysis was done using one-way ANOVA. (*) denotes statistically different (p < 0.05) from medium control. (+) denotes statistically significant (p < 0.05) from rgIFNγ-induced phagocytosis values.