FIGURE 2.

Compounds A, B, and C are potent TLR2-1 agonists. SW620 cells were co-transfected with vectors encoding the indicated TLRs, an IL-8-driven firefly luciferase gene, and a Renilla luciferase control. Two days after transfection, cells were stimulated with different concentrations of compound A, B, or C for 6 h, and cell lysates were analyzed for Dual-Luciferase activity. After correcting for transfection efficiency using Renilla luciferase, all values were normalized to those of unstimulated cells transfected with empty FLAG-CMV vector (CMV). Error bars represent the S.D. of three independent wells stimulated with the indicated concentration of agonist.