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Effect of Tween 20 on MFP activity and substrate profile. A, AtMFP2 dehydrogenase activity was assayed with increasing concentrations of Tween 20. B, the dehydrogenase activities of AtMFP2 (black) and AtAIM1 (gray) with C14-CoA and C16-CoA in the presence of 0.1% (w/v) Tween 20 were determined. The insert illustrates 2-trans-enoyl-CoA production of each substrate. Conditions in both assays were the same as in Fig. 4.
