FIGURE 6.

BSA effect on MFP activity and substrate profile. A, AtMFP2 was titrated with BSA, and dehydrogenase activity was determined. B, substrate was prepared in the presence of 6 μm delipidated BSA, and hydratase activity was subsequently determined, C. D, dehydrogenase activity was determined with 150 times more MFP in the C16- and C18-CoA wells compared with 0.5 nm in the C14-CoA well. The data are not normalized to μm enzyme to show residual long chain dehydrogenase activity at high enzyme concentrations. Conditions otherwise are as described for Fig. 4.