TABLE 2.
Packaging velocities measured by optical tweezer assays and catalytic activities of wild-type and mutant terminase enzymes
NE is not examined.
Enzyme | Packaging Vmax | ATPase activity |
---|---|---|
Wild-type | 580 ± 120 bp/s | 81 ± 8 min−1 |
G212S | 195 ± 66 bp/s | 2.0 ± 0.2 min−1 |
T194M | 82.0 ± 20.7 bp/s | 0.6 ± 0.2 min−1 |
T194M/F156Y | 56.9 ± 8.9 bp/s | NE |
T194V | 420 ± 143 bp/s | NE |