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. 2010 Jun 4;285(31):24282–24289. doi: 10.1074/jbc.M110.129395

TABLE 2.

Packaging velocities measured by optical tweezer assays and catalytic activities of wild-type and mutant terminase enzymes

NE is not examined.

Enzyme Packaging Vmax ATPase activity
Wild-type 580 ± 120 bp/s 81 ± 8 min−1
G212S 195 ± 66 bp/s 2.0 ± 0.2 min−1
T194M 82.0 ± 20.7 bp/s 0.6 ± 0.2 min−1
T194M/F156Y 56.9 ± 8.9 bp/s NE
T194V 420 ± 143 bp/s NE