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Growth dependence of the MD001 and MD003 mutants toward nisin and d-lactate. The L. lactis MD001 (A) and MD003 (B) mutants were cultured in M17-glucose in the presence and absence of d-Lac (15 mm) and nisin (0.5 ng/ml), shown as closed and open triangles, respectively, and in the presence of nisin (0.5 ng/ml) without d-Lac (open circles). Growth of the cultures was followed by measuring the A600 nm.
