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. 2010 Jun 4;285(31):24270–24281. doi: 10.1074/jbc.M110.125658

FIGURE 3.

FIGURE 3.

Suppression of Arf1 or Arf6 decreases wound closure of 3T3-L1 cells. A, in the wound healing assay 3T3-L1 cells were transfected with an siRNA for control (luciferase), Arf1, or Arf6. 3T3-L1 cell monolayers were wounded by scraping and observed microscopically at 0 and 6 h. The rate of healing was calculated based on the width of the wound. Data were evaluated with one-way ANOVA (*, p < 0.01). B, cell lysates were immunoblotted with an antibody against Arf1, Afr6, or actin.

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