TABLE 2.
Comparison of the reported Vmax values of human erythrocyte enzymes with those included in the model
All values are in mmol(liter of erythrocytes)−1 h−1 ± S.E. where available. GSGSase, glutathione synthetase; γ-GCTase, γ-glutamylcyclotransferase; NACase, N-acetylcysteine deacetylase; ALATase, alanine aminotransferase; GLNase, glutaminase.
| Enzyme | Reported values | Model values | Reference No. |
|---|---|---|---|
| GCL | 5.1 | 14.0 | 74 |
| 27.8–33.7 | 58 | ||
| 122 ± 6.3 | 75 | ||
| GSHSase | 5.1 | 17.5 | 11 |
| 4.50–9.91 | 58 | ||
| 20.4 ± 1.6 | 75 | ||
| γ-GCTase | 2.24 | 2.24 | 11 |
| GSSGR | 123 ± 22 | 132 | 12 |
| NACase | 0.110 ± 0.003 | 0.126 | 45 |
| ALATase | 0.917 ± 0.290a | 0.861 | |
| GLNase | 5.08a | 5.12 | 76 |
| 10.5 |
a Vmax values for alanine aminotransferase and glutaminase were measured using 1H NMR spectroscopy to follow the reactions in concentrated hemolysates (results not shown).