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. 2010 May 24;285(31):23557–23567. doi: 10.1074/jbc.M109.067017

TABLE 2.

Comparison of the reported Vmax values of human erythrocyte enzymes with those included in the model

All values are in mmol(liter of erythrocytes)−1 h−1 ± S.E. where available. GSGSase, glutathione synthetase; γ-GCTase, γ-glutamylcyclotransferase; NACase, N-acetylcysteine deacetylase; ALATase, alanine aminotransferase; GLNase, glutaminase.

Enzyme Reported values Model values Reference No.
GCL 5.1 14.0 74
27.8–33.7 58
122 ± 6.3 75
GSHSase 5.1 17.5 11
4.50–9.91 58
20.4 ± 1.6 75
γ-GCTase 2.24 2.24 11
GSSGR 123 ± 22 132 12
NACase 0.110 ± 0.003 0.126 45
ALATase 0.917 ± 0.290a 0.861
GLNase 5.08a 5.12 76
10.5

a Vmax values for alanine aminotransferase and glutaminase were measured using 1H NMR spectroscopy to follow the reactions in concentrated hemolysates (results not shown).