FIGURE 3.

Western blot analysis from co-immunoprecipitation studies. Lanes contained the following: lane 1, elution 1 from MFP beads; lane 2, load onto MFP beads; lane 3, peroxisomal lysate; lane 4, load onto KAT beads; lane 5, elution 3 from KAT beads; lane 6, elution 2 from KAT beads; lane 7, elution 1 from KAT beads; lane 8, molecular weight marker. A, Western blot developed with primary antibody against KAT. B, Western blot developed with primary antibody against MFP. A band is seen for MFP from the KAT2 pulldown suggesting a complex (black circle). Elution steps were performed only after extensive washing.