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. 2010 Jun 1;285(31):23994–24002. doi: 10.1074/jbc.M110.144063

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Double, triple, and quadruple cysteine KCC2 mutants are transport-inactive. The ⁸⁶Rb⁺ uptake of KCC2_wt and compound mutants were measured in transfected HEK-293 cells. All double cysteine (A) and triple and quadruple cysteine (B) KCC2 mutants were transport-inactive because their transport activity showed no significant difference from the empty vector. Plots depict mean of three experiments ± S.D. (error bars). One-way ANOVA of all determinants demonstrated a significant multivariate effect for the double mutants (F = 31.1; p < 0.001) and for the triple and quadruple mutants (F = 3316.99; p < 0.001). ANOVA without KCC2_wt revealed no significant difference between the double mutants (F = 0.16; p = 0.98) or the triple and quadruple mutants (F = 1.64; p = 0.17) compared with mock-transfected cells.