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. 2010 Jun 1;285(31):23994–24002. doi: 10.1074/jbc.M110.144063

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FIGURE 6. — The large extracellular loop of KCC2 but not of KCC4 requires the parental backbone. HEK-293 cells were assayed for ⁸⁶Rb⁺ uptake 48 h after transfection with the constructs indicated below the columns. An empty vector was used for mock transfection. A, KCC2_wt (white bars), KCC4_wt (black bars), and the chimera KCC2_2-4-2 (striped bars) showed transport activity. The transport activity of KCC2_wt and the chimera KCC2_2-4-2 is comparable. KCC2_2-4q-2 displayed strongly reduced transport activity compared with KCC2_wt (p < 0.001). B, KCC4_4-2q-4 and KCC4_4-2-4 are transport-inactive. The plot depicts mean of three experiments ± S.D. ***, p < 0.001; *, p < 0.05; n.s., not significant.