Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Concentration-response profile for inhibition of KCC2, KCC4, and KCC22-4-2 by furosemide. Transfected HEK-293 cells with KCC2, KCC4, and KCC22-4-2 were exposed to increased furosemide concentration from 2 to 2000 μm in the preincubation and uptake buffer. The activity of untreated cells of the respective KCC was normalized to 100%. IC50 values were determined by using logarithmic regression analysis. The plot depicts the mean of four experiments ± S.D. ***, p < 0.001.
