TABLE 1.
Description of complex KCC mutants
| Clone abbreviation | Comment | KCC2 residues (GenBankTMNM_134363) | KCC4 residues (GenBankTMNM_011390) | Mutation |
|---|---|---|---|---|
| KCC2q | Quadruple mutated extracellular loop | 1–1116 | C287S,302L,C322S,C331L | |
| KCC22-4-2 | Extracellular loop of KCC4 | 1–276, 399–1116 | 298–418 | |
| KCC22-4q-2 | Quadruple mutated extracellular loop of KCC4 | 1–276, 399–1116 | 298–418 | C308S,C323L,C343S,C352L |
| KCC4q | Quadruple mutated extracellular loop | 1–1103 | C308S,C323L,C343S,C352L | |
| KCC44-2–4 | extracellular loop of KCC2 | 277–402 | 1–297, 423–1103 | |
| KCC44-2q-4 | Quadruple mutated extracellular loop of KCC2 | 277–402 | 1–297, 423–1103 | C287S,302L,C322S,C331L |