FIGURE 2.

Two-dimensional TLC analysis of membrane lipids extracted from MRSA-16 grown to mid-logarithmic phase (A₆₀₀ = 0. 7) in the absence (A) or presence (B) of 12.5 μg/ml of ECg. The growth medium (MH broth) contained 18.5 KBq/ml of [³²P]orthophosphate. The spots were visualized by autoradiography, and their intensity was quantified using a Bio-Rad Molecular Imager FX; the data are presented in the bottom panel. CL, cardiolipin; lysoPG, lysophosphatidylglycerol. The LPG total is the sum of values obtained from spots LPG and LPG-2. Each of the minor phospholipid components from ECg-free and ECg-containing cultures constituted less than 1% of total lipid extracted. The values given are the percentages of total lipid extracted ± S.D. (n = 9).