FIGURE 2.

NALT uptake of nanoparticles prepared with and without plx. A, 20 μl of nanoparticle dispersion (in PBS) was given to each nostril (20 μg of protein per animal), and the NALT tissue was dissected and stained for UEA-1. Confocal images were recorded in three different areas of the same NALT where UEA-1-positive M cells were visible. Each symbol denotes the number of particles taken up in each 7850 μm² area that was analyzed in each NALT tissue dissected, with three mice for each condition. Uptake of fluorescent PLGA nanoparticles show increased NALT uptake when targeted protein (HA-ts-HT-CPE) is incorporated, with a further enhancement when plx is also incorporated.